Phenylacetate-coa
WebIn enzymology, a phenylacetate—CoA ligase is an enzyme (EC 6.2.1.30) that catalyzes the chemical reaction. ATP + phenylacetate + CoA AMP + diphosphate + phenylacetyl-CoA. … WebJul 21, 2010 · Phenylacetyl-CoA is the substrate of a presumed multicomponent oxygenase, PaaABCDE. This oxygenase is a key enzyme of the pathway, proposed to be responsible for the introduction of oxygen into the aromatic ring of phenylacetyl-CoA ( 6, 14, 21 ). First we … We would like to show you a description here but the site won’t allow us.
Phenylacetate-coa
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WebApr 21, 2006 · Phenylacetate derives primarily from phenylalanine, but it is also formed in degradation pathways that target a variety of environmental aromatics of natural and synthetic origin and, thus, is the central element of an important catabolon ( 20, 21, 22 ). WebDownload alignment. phenylacetate-CoA oxygenase, PaaG subunit. Phenylacetate-CoA oxygenase is comprised of a five gene complex responsible for the hydroxylation of phenylacetate-CoA (PA-CoA) as the second catabolic step in phenylacetic acid (PA) degradation. Although the exact function of this enzyme has not been determined, it has …
WebAug 10, 2010 · Although a phenylacetate metabolic gene cluster had been identified, the underlying biochemistry remained largely unknown. Here we elucidate the catabolic pathway functioning in 16% of all... WebNov 1, 2008 · The phl gene, encoding a PCL (phenylacetate–CoA ligase), was cloned in Escherichia coli as a maltose-binding protein fusion and the biochemical properties of the enzyme were characterized. The...
WebApr 26, 2011 · Phenylacetate is first activated through the addition of CoA. The phenylacetyl-CoA is then converted to epoxide by a multicomponent monooxygenase. The aromatic … WebJul 1, 2024 · Phenylacetate is converted into phenylacetyl-CoA by phenylacetate-CoA ligase, and this product then undergoes a series of degradative steps through ring hydroxylation, ring opening, and β-oxidation type degradation [122]. The PaaI thioesterases release CoA from phenylacetyl-CoA, as well as mono- and dihydroxylated compounds 4 ...
WebApr 21, 2006 · The acyl-CoA ligand is bound at the protomer-protomer interface with the acyl-thioester moieties inserted into the core of the dimer. The ligand CoA units rest on …
http://eawag-bbd.ethz.ch/pac/pac_map.html jennifer mathenyWebphenylacetic acid: [ fen″il-ah-se´tik ] a catabolite of phenylalanine ; it is excessively formed and excreted, sometimes conjugated with glutamine , in phenylketonuria . pac rhythmsWebJun 1, 1993 · The enzyme catalysing the first step in the anaerobic degradation pathway of phenylacetate was purified from a denitrifying Pseudomonas strain KB 740. It catalyses the reaction phenylacetate+CoA+ATP → phenylacetyl-CoA+AMP+PPi and requires Mg2+. Phenylacetate-CoA ligase (AMP forming) was found in cells grown anaerobically with … pac rim leasingWebMethyl phenylacetate undergoes decomposition on photolysis in methanol. Methyl phenylacetate acts as acylating agent and causes the enantioselective acylation of beta … jennifer matash macomb miWebNational Center for Biotechnology Information pac rim log homesWeb2-phenylacetate + ATP + CoA = AMP + diphosphate + phenylacetyl-CoA 1 publication This reaction proceeds in the forward direction. 1 publication EC:6.2.1.30 ( UniProtKB ENZYME Rhea) Source: Rhea 20956 Hide Rhea reaction 2-phenylacetate CHEBI:18401 zoom + ATP CHEBI:30616 zoom + CoA CHEBI:57287 zoom = AMP CHEBI:456215 zoom + diphosphate jennifer mathers mchenryWebMar 10, 2015 · The phenylacetate-CoA binds to the PaaA subunit in a hairpin conformation within a ~20Å deep pocket in the protein. The adenine and phenyl moieties at the two ends of the substrate point in the same direction and the phosphates are at the hairpin bend near the protein surface (Figure 2A). The entrance to the substrate-binding pocket is ... pac rim medical hawaii