Webb高彩霞团队改造并比较了三组植物引导编辑系统（plant prime editor systems，PPEs），它们分别为PPE2,PPE3和PPE3b。 其中，PPE2对应上文刘如谦团队的PE2，包含融合逆转录酶M-MLV-RT的nCas9（H840A），和一个包含PBS和反转录模板的pegRNA。 PPE3对应于上文的PE3，在PPE2的基础上添加了一个可以靶向并指导切割非编辑链sgRNA。 PPE3b则 … http://www.plantgenomeediting.net/

(PDF) Precise base editing in rice, wheat and maize with a Cas9 ...

Webb21 feb. 2024 · Cas9 protein (1 μg) and sgRNA (1 μg) were mixed with the purified target DNA (100–150 ng) in Cas9 reaction buffer (20 mM HEPES, pH 7.5, 150 mM KCl, 10 mM MgCl 2, 0.5 mM DTT) in a total volume of 20 μl, followed by digestion at 37 °C for 0.5–1 h. WebbpH-nCas9-PBE质粒价格1200元，pH-nCas9-PBE(爱迪基因 98163 )质粒图谱（map），质粒序列(Sequence)见下文，质粒抗性为卡那霉素。pH-nCas9-PBE质粒是植物表达；水稻。 pa schools in illinois without gre

Optimized prime editing in monocot plants using PlantPegDesigner and

Webb3 jan. 2013 · Fig. 1 Genome editing in human cells using an engineered type II CRISPR system. ( A) RNA-guided gene targeting in human cells involves coexpression of the … WebbPlasmid pCre-AntiDsRed from Dr. James Birchler's lab contains the insert AntiDsRed Cre recombinase and is published in Unpublished This plasmid is available through Addgene. http://www.solentcgcom.com/139674/ ting speed test centennial