Peasy t3

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August undefined, 2024

Webplasmids pEASY-T3 and pET-30a were used for cloning, sequencing, and expression, respectively. The substrate D-glucose from Sigma-Aldrich was used for the enzyme activity assay. Fructose and other chemicals were of analyti-cal grade and commercially available. 2.2.SequenceAnalysisandGeneCloning.TheC.besciiDSM Web将稳定表达pCPK6∷GUS的拟南芥T3转基因种子播于全营养培养基(CK)，4 ℃低温处理3 d，竖直光照培养7 d，分别移至CK和Ca2+、Fe2+、Mg2+、Zn2+和Mn2+缺乏的固体培养基上。继续竖直光照培养7 d后，采用组织化学法定位检测GUS活性。

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Web大肠杆菌（Escherichia coli） K12基因组中,仅dctA基因编码1个在有氧条件下负责摄取四碳-二羧酸的转运蛋白.为鉴定DctA能否… WebpEASY®- T3 Cloning Vector provides dual- Eco RI and dual- Not I restriction sites. It is designed for cloning and sequencing Taq- amplified PCR products 3’A-overhangs). The cloned insert can be released from a single enzyme digestion. The vector is suitable for T7 RNA polymerase-mediated In vitro transcription using our T7 High-Efficiency ... irs code for property management

[Construction of quantitative real-time PCR detection system of ...

WebNov 12, 2013 · The amplified fragments were subsequently purified using the EasyPure Quick Gel Extraction Kit (TransGen) and cloned into pEASY-T3 plasmid using the pEASY T3 Cloning kit (TransGen) according to the manufacturer's recommendations. To identify sequencing errors, at least three clones of each sample were sequenced (SunbioTech). WebSep 6, 2024 · pEASY-Blunt Cloning vector to obtain the recombinants, and then transformed the recombinants into ... The PCR product of BtabOBP3 was cloned into a pEASY-T3 vector, and then the target fragment ... WebOct 1, 2024 · Then the fragments were cloned into pEASY®-T3 Cloning Vector and transformed into Trans1-T1 competent cell using pEASY®-T3 Cloning Kit (TransGen Biotech Co., Ltd., Beijing, China). The positive plasmid were obtained according to blue/white selection and identified by colony PCR and sequencing. portable small homes

pEASY-T3 Cloning Kit, View Cloning vector, TransGen-pEASY

拟南芥CPK6在钙离子信号转导过程中的生理功能研究_百度文库

WebTriiodothyronine, also known as T3, is one of the two main hormones your thyroid gland releases into your bloodstream. Your thyroid also produces thyroxine, also known as T4 … WebMay 29, 2024 · Therefore, pEASY T3-ydhE was used for the positive control of a H + -coupled norfloxacin efflux transporter, whereas pEASY T3-nhaD was used for the positive … irs code for religious organizationsWebAug 1, 2024 · The program was set on a Bio-Rad T100 Thermal Cycler as follows—94°C 2 min; 94°C 30 s, 58°C 30 s, 72°C 2 min, 27 cycles; 72°C 2 min. Target products were purified by using the Wizard® SV Gel and PCR Clean-Up System (Promega, Madison, USA), and then ligated to the pEASY-T3 vector (TransGen, Beijing, China) for sequencing (Invitrogen, … portable small homes for sale

"WebA T3 (triiodothyronine) test helps diagnose thyroid conditions, particularly hyperthyroidism (overactive thyroid). Your thyroid is a small, butterfly-shaped gland located at the front of your neck under your skin. It’s a part of your endocrine system. Triiodothyronine, also known as T3, is one of the two main thyroid hormones. " - Peasy t3

Peasy t3

WebThe pEASY-T3-VP7 plasmid was digested using HindⅢ and BamHI restriction endonucleases, then recombined into the prokaryotic expression vector pET32a. The … WebFree Trial pEASY-Blunt Simple (linearized) Cloning vector for blunt-ended PCR products, with blue-white selection, kanamycin and ampicillin resistance markers, and a T7 promoter but …

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WebpEASY-T3 (linearized) TA cloning vector that allows the cloned PCR product to be excised with EcoRI or NotI. Sequence Author: TransBionovo (TransGen Biotech) Open in … WebThe sequence result of the pEasy-T3 cloned plasmid of E. longifolia revealed that the rbcL and ITS2 fragments have a sequence length of 1346 and 313 bp, respectively. The sequences generated for rbc L (FDB) and ITS2 have 99% similarities with those in …

WebPicoGreen was used to measure the DNA concentration of the plasmid reference molecules. Two sets of samples containing 1% or 0.1% (w/w) pEasy-T3-16A or pEasy-T3-16S mixed … WebThe PCR products were cloned into the vector pEASY-T3 and sequenced. Based on the known sequences, six specific primers were designed to obtain the 59 and 39 flanking regions (data not shown). The gene fragments were assembled and subject to …

WebNov 15, 2024 · The fragments amplified through 5’- and 3’-RACE were inserted into the vector PEASY-T3 and sequenced. The full-length sequence of PtHMGR was obtained by aligning these sequences. Finally, the ORF of PtHMGR was amplified with gene-specific primers (Table S1: ORF-HMGR-F and ORF-HMGR-R), cloned into the vector PEASY-T3, and …

WebOct 11, 2024 · Plasmids T3, pEASY -T3 and pET -30a were availed for cloning and expression, and the pGBKT7 and pGADT7 plasmids were applied in yeast two-hybrid assays ( Wang et al., 1995; Guo et al., 2007 ). The pB1H1 and pH3U3 plasmids were used as bait and expression vectors, and the pSUP202 vector was used to construct the gene …

WebJul 10, 2024 · T3 is part of Future US Inc, an international media group and leading digital publisher. Visit our corporate site © Future US, Inc. Full 7th Floor, 130 West 42nd Street … irs code for roth conversionWebNov 27, 2024 · pEASY-T3 vector. 7. T4 DNA ligase. 8. Escherichia coli Trans1-T1 cells. 2.2 Phytase Production. 1. pPIC9γ vector. 2. Restriction endonucleases EcoRI, NotI, and BglII. … irs code for real estate rentalsWebJan 15, 2024 · The modified gene is recovered, connected with the vector pEASY-T3, and sequenced. Example 2 Preparing the Phytase Variants and Measuring their Activity The modified gene encoding the phytase variants were inserted into expression vector pET-22b (+), and transformed into E coli. irs code for retail storeWebpEASY ®-T3 Cloning Vector provides dual-EcoR I and dual-Not I enzyme digestion sites. It is designed for cloning and sequencing Taq amplified PCR products. The cloned insert can … portable small fridge displayWebApr 21, 2024 · The white rot fungus Irpex lacteus is one of the most potent fungi in degradation of lignocellulose and xenobiotics. Two manganese peroxidases ( Il MnP1 and Il MnP2) from I. lacteus CD2 were over-expressed in Escherichia coli and successfully refolded from inclusion bodies. Both Il MnP1 and Il MnP2 oxidized the phenolic compounds … irs code for tattoo artistWeb15 hours ago · Rejuvenating your leggy and stretched-out succulents is so easy. 1. Remove the whole plant from its pot. 2. Carefully twist off any dead or dying leaves. 3. Continue by … irs code for pressure washingWebpEASY ® - T3 Cloning Vector provides dual EcoR I and dual Not I enzyme sites. It is designed for cloning and sequencing Taq-amplified PCR products. The cloned insert can … portable small homes mother in law